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PUMA + / + och PUMA - / - kullkamrater på C57BL / 6-bakgrund (F10) 48 och Lgr5-EGFP ( Lgr5-EGFP-IRES-creERT2 ) -möss 25 har beskrivits. Möss utsattes för 

Submit. Contact us Gene-editing proposal Back to top Heterozygous mice harbor an Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function and expresses EGFP and CreERT2 fusion protein. When these mice mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Lgr5-expressing cells of the offspring. When using the Lgr5-EGFP-IRES-creERT2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008875 in your Materials and Methods section. AX10 (Standard) Animal Health Reports Facility Barrier Level Descriptions Pricing & Availability Available Live mice available in varying quantities.

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Rosa26RlacZ mice were obtained from Jackson Labs. All animal experiments were approved by the Animal Experimentation Committee of the Royal Dutch Academy of Science and the Institutional Animal Care and Use Committee of Singapore. By using the knock-in allele Lgr5 EGFP-Ires-CreERT2, we have shown that the cell surface receptor Lgr5 (leucine-rich repeat–containing heterotrimeric guanine nucleotide–binding protein–coupled receptor 5) marks normal tissue stem cells in stomach, small intestine, colon, and hair follicles . with Lgr5-eGFP-IRES-CreERT2 (Lgr5-Cre) mice. To this end, tamoxifen (Tam) was administered to 8-10-week-old mice. After three consecutive days of Tam treatment, double immunofluorescence staining showed that some Lgr5+ antral stem cells lacked E-cadherin expression in Lgr5-Cre;Cdh1fl/fl mice (Figure 2A).

Lgr5-EGFP-IRES-CreERT2: Gene: Lgr5 Location: Chr10:115450314-115587780 bp, - strand Genetic Position: Chr10, 63.51 cM Mutation origin: Germline Transmission: Earliest citation of germline transmission: J:212415: Parent Cell Line: Not Specified (ES Cell) Strain of Origin: C57BL/6:

Cre enzyme activity was induced by intraperitoneal injections of tamoxifen (2 mg/mouse). Murine small intestines with Lgr5-eGFP-IRES-CreERT2 (Lgr5-Cre) mice.

cells in Lgr5-eGFP-IRES-CreERT2 mice. In contrast, only 1.7 ± 0.30% of Bmi1+ ISCs, identified by the crypt Rosa-YFP+ signal after 1.5-d tamoxifen exposure in Bmi1-CreER; Rosa26-YFP mice, incorporated EdU (Fig. 1 A–F and N). To examine the relative contribution of Lgr5 vs. Bmi1 ISCs to tissue regeneration under steady-state conditions

Lgr5EGFP-Ires-CreERT2/Apc fl/flmice with the R26R-Confetti strain.

2 a) in the majority (69.9%) of the villi (Additional file 3 : Figure S2A).
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To induce the expression of the reporter gene, a single  Mus musculus, C57BL/6J, ET993, 3 to 9 (month), young, female, Lgr5-EGFP- IRES-creERT2, epithelium of small intestine, intestinal crypt stem cell, young  1 Aug 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti  21 Jun 2012 Retracing of Lgr5+ adenoma cells. (A) Apc-deficient adenomas were allowed to develop for 24 days in Lgr5-EGFP-Ires-CreERT2/Apcfl/fl/R26R  Also Known As:Lgr5-EGFP-IRES-creERT2. Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function  were generated by crossing Lgr5–EGFP–ires–CreERT2 and. Klf5 flox/flox mice ( 4, 29). Because Cre–ERT2 fusion gene activity is inducible following tamoxifen  In 4 Lgr5-EGFP-IRES-CreERT2 Kras LSL-G12D mice studied between 2 and 8 months after tamoxifen.

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life. 查看. Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute. 2019-12-31 Lgr5-EGFP-IRES-CreERT2: Gene: Lgr5 Location: Chr10:115450314-115587780 bp, - strand Genetic Position: Chr10, 63.51 cM Mutation origin: Germline Transmission: Earliest citation of germline transmission: J:212415: Parent Cell Line: Not Specified (ES Cell) Strain of Origin: C57BL/6: Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements.
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Heterozygous mice harbor an Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function and expresses EGFP and CreERT2 fusion protein. When these mice mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Lgr5-expressing cells of the offspring.

To induce Cre recombinase in Lgr5 EGFP-Ires-CreERT2 ; Rosa26-LacZ mice , tamoxifen (Sigma) was dissolved in corn oil and injected intraperitoneally from postnatal day 3 to 4 (P3–P4; 0.3 mg/g body weight). Young (2–3-month-old) and old (> 19-month-old) Lgr5-EGFP-IRES-creERT2 mice (The Jackson Laboratory) were total-body irradiated with or without 10 Gy of X-rays and sacrificed 8 h later. Swiss roll sections of paraffin-embedded intestines were prepared and immunohistochemically analyzed. May 8, 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-.